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Application of peristaltic pump in tissue perfusion

2021-05-29 16:05:41
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Underwater concrete and dispensing culture solution, buffer solution or other liquids are common daily tasks for all laboratories. It is an important challenge to apply pumps to solve the problem of liquid to ensure high precision migration of liquid and maintain zero pollution. Peristaltic pump is a kind of high-precision liquid transmission and processing equipment, because it has the characteristics of high total flow linearity, simple practical operation, low shear stress, and can prevent cross environmental pollution in the middle of the liquid, it is widely used in the laboratory. After that, I will share with you how Xialaizhou peristaltic pump can help complete the accurate transmission of liquid and solve the actual operation in different applications in the laboratory.

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Instrumental perfusion is one of the experimental ways for medical academies and scientific research institutes to carry out pharmaceutical or physiological research. For example, when scientific research paraffin sectioning brain has nerve cells all over the area, in order to better ensure the physiological specificity of human organs in vitro during the whole process of the experiment, it is necessary to continuously transport cell culture solution containing carbon dioxide to paraffin sectioning institutions or human organs, The whole process is called mechanism perfusion. The institutional perfusion test can be suitable for the physiological and pharmaceutical scientific research of human organs such as capillaries, sarcoplasmic reticulum, heart, coronary arteries, intestines, etc.

When conducting mechanism perfusion, Laizhou peristaltic pump is generally used together with patch clamp equipment to deliver CO2 containing cell culture solution to paraffin sectioning mechanism or underwater concrete of human organs, so that relevant tests can be carried out smoothly. The peristaltic pump can be adjusted and operated according to the set irrigation mode, irrigation rate, total irrigation flow, etc., incorporating different scientific research requirements and institutional building requirements.

The legend is the schematic diagram of the mechanism perfusion system software. The peristaltic pump transmits the perfusion fluid with carbon dioxide to the conversion valve, which selects the appropriate perfusion fluid and inputs it into the perfusion tank. The paraffin sectioning mechanism/human organ in the perfusion tank maintains a stable physiological condition under the effect of the perfusion fluid, and the liquid after perfusion is discharged from the perfusion tank according to the peristaltic pump. The electric grade needle loaded on the human organ of the paraffin sectioning mechanism is used to record the information content of electrophysiological needs.

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